New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples

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New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples.

Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

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Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.

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A quantitative TaqMn Real-Time PCR assay was developed and its diagnostic value on human serum and livestock samples were evaluated. Brucella species could be distributed through communities as a biological agent. Rapid detection of biological threat agents is critical for timely therapeutic administration. Quantitative real-time PCR provides a rapid, sensitive and specific tool for molecular i...

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Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2008

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.02302-07